Alkaline lysis or alkaline extraction is a method used in molecular biology to isolate plasmid DNA from bacteria. Bacteria containing the plasmid of interest...
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Plasmid preparation (section Alkaline lysis)
several methods for cell lysis, including alkaline lysis, mechanical lysis, and enzymatic lysis. The most common method is alkaline lysis, which involves the...
17 KB (1,955 words) - 13:31, 10 June 2024
Look up lysis or -lysis in Wiktionary, the free dictionary. Lysis is the breaking down of the membrane of a cell. Lysis may also refer to: Lysis (dialogue)...
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Restriction map (section Rapid Denaturation and Renaturation of a crude DNA preparation by alkaline lysis of the cells and subsequent neutralization)
produced by all three digests In this technique the cells are lysed in alkaline conditions. The DNA in the mixture is denatured (strands separated) by...
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with other resuspension buffers and lysis buffers to release DNA from cells, often as part of the alkaline lysis method of purifying plasmid DNA from...
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zwitterionic detergents such as CHAPS Part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer) in DNA extraction Reducing surface tension...
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nephrolithiasis, are not features of tumor lysis syndrome.[citation needed] Lactic acidosis. Pretreatment spontaneous tumor lysis syndrome. This entity is associated...
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gel electrophoresis - agarose gel - akaryocyte - Alagille syndrome - alkaline lysis - allele - amino acids - amino terminus - amp resistance - amplification...
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Hydrolysis (redirect from Alkaline hydrolysis)
Hydrolysis (/haɪˈdrɒlɪsɪs/; from Ancient Greek hydro- 'water' and lysis 'to unbind') is any chemical reaction in which a molecule of water breaks one...
18 KB (2,240 words) - 13:05, 26 August 2024
Comet assay (section Lysis)
encapsulation of cells in a low-melting-point agarose suspension, lysis of the cells in neutral or alkaline (pH>13) conditions, and electrophoresis of the suspended...
20 KB (2,499 words) - 09:42, 11 March 2024