Third-generation sequencing (also known as long-read sequencing) is a class of DNA sequencing methods which produce longer sequence reads, under active...
33 KB (3,988 words) - 15:39, 3 April 2024
parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Some of these technologies emerged between 1993...
29 KB (3,019 words) - 01:30, 11 December 2023
High-throughput sequencing, which includes next-generation "short-read" and third-generation "long-read" sequencing methods, applies to exome sequencing, genome...
129 KB (14,413 words) - 10:39, 30 May 2024
in 1986. More recently, higher volume Sanger sequencing has been replaced by next generation sequencing methods, especially for large-scale, automated...
27 KB (3,261 words) - 11:07, 15 June 2024
Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Using...
38 KB (4,564 words) - 05:30, 1 April 2024
Read (biology) (category DNA sequencing)
produced by Sanger sequencing. Beginning in the 2010s, revolutionary new technologies ushered in the Third-Generation Sequencing era (TGS). TGS is a...
16 KB (2,055 words) - 12:21, 21 January 2024
assembled back into chromosomes using only short reads provided by next-generation- sequencing technologies (NGS), and therefore most plant genome assemblies available...
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Hybrid genome assembly (section Hybrid error correction and de novo assembly of single-molecule sequencing reads)
Resolving these tandem repeats can be accomplished by utilizing long third generation sequencing reads, such as those obtained using the PacBio RS DNA sequencer...
21 KB (2,951 words) - 11:31, 30 April 2024
Genomics (section Early sequencing efforts)
on the technology used. Third generation sequencing technologies such as PacBio or Oxford Nanopore routinely generate sequencing reads >10 kb in length;...
78 KB (7,539 words) - 18:04, 25 May 2024
Second-generation sequencing has yielded many draft genomes (close to 90% of bacterial genomes in GenBank are currently not complete); third-generation sequencing...
34 KB (4,428 words) - 21:11, 25 April 2024