In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds...
22 KB (2,535 words) - 04:02, 28 October 2023
Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses...
7 KB (842 words) - 06:09, 29 November 2023
Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal...
5 KB (536 words) - 14:58, 26 August 2023
Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger...
36 KB (4,657 words) - 08:51, 23 July 2024
Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made...
27 KB (3,147 words) - 04:36, 16 May 2024
Genome editing (redirect from Genome editing with engineered nucleases)
current nuclease-based gene editing platforms but its use was limited by low efficiencies of editing. Genome editing with engineered nucleases, i.e. all...
78 KB (9,362 words) - 13:30, 27 July 2024
MNase-seq (redirect from Micrococcal nuclease sequencing)
MNase-seq, short for micrococcal nuclease digestion with deep sequencing, is a molecular biological technique that was first pioneered in 2006 to measure...
39 KB (4,420 words) - 19:10, 15 May 2024
Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for...
2 KB (229 words) - 19:29, 29 August 2023
Surveyor nuclease assay is an enzyme mismatch cleavage assay used to detect single base mismatches or small insertions or deletions (indels). Surveyor...
26 KB (3,176 words) - 03:46, 22 January 2024
Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, Kamchatka crab) hepatopancreas that displays a...
2 KB (178 words) - 20:57, 9 July 2024