A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with...
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specific target protein. Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry and immunocytochemistry, where antibodies...
49 KB (6,081 words) - 06:04, 30 December 2024
probes. A dot blot is a special case of any of the above blots where the analyte is added directly to the blotting matrix (and appears as a "dot") as opposed...
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probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler dot blot assay. It is a common tool used in genetic testing...
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chemically modified paper filters or microporous membrane filters. In a dot blot, macromolecules are applied directly to the matrix. Macromolecules can...
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oligonucleotide (ASO) probes, a process that can often be done by a simple dot blot. Amplified fragment length polymorphism (AFLP) RAPD STR analysis Saiki...
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antigen-antibody reactions are being explored, such as immuno-PCR. A rapid dot-blot ELISA test is also available for quick, effective, and economical on-site...
44 KB (4,583 words) - 04:47, 16 January 2025
antigen (with a test such as ELISA or antigen microarray assay) or immuno-dot blot. The most productive and stable clone is then selected for future use....
48 KB (4,972 words) - 04:27, 21 December 2024
affinity purification Protein:protein interaction studies Colony blot, dot blot, Western blot and ELISA Screening for positive expression clones Immunocytochemistry...
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Railroader. On a calendar he would mark a dot for each hour worked, half a dot for a half hour, and a blot for anything thing that seemed like 3 hours...
36 KB (2,833 words) - 03:01, 6 August 2024