In spectroscopy, an isosbestic point is a specific wavelength, wavenumber or frequency at which the total absorbance of a sample does not change during...
7 KB (837 words) - 10:28, 20 May 2024
maxima) for the individual species. None of the wavelengths may be an isosbestic point for a pair of species. The set of the following simultaneous equations...
6 KB (760 words) - 18:58, 26 June 2024
4-nitrophenol has a weak absorbance at 405 nm (ε = 0.2 mM−1 cm−1). The isosbestic point for 4-nitrophenol/4-nitrophenoxide is at 348 nm, with ε = 5.4 mM−1...
9 KB (935 words) - 12:50, 5 February 2024
TNP-ATP (section pKa and isosbestic point)
shifts in neutral pH. Spectroscopic superposition indicated TNP-ATP’s isosbestic point to be 339 nm. At low concentrations of TNP-ATP (≤1 μM), fluorescent...
16 KB (2,206 words) - 04:04, 22 June 2024
the maximum absorption and isosbestic points of the indicator. The isosbestic point of GCaMP is the calcium-independent signal, approximately 405-415 nm...
38 KB (4,383 words) - 06:43, 5 November 2023
characteristic blue-green color. The acid and basic forms of this dye have an isosbestic point in their UV-Visible spectrum, around 515 nm, indicate that the two...
8 KB (545 words) - 17:55, 9 February 2024
in situ to give oxidized, ring-opened products. Fluorescein has an isosbestic point (equal absorption for all pH values) at 460 nm. Many derivatives of...
26 KB (2,512 words) - 15:23, 31 May 2024
wavelengths are selected, with one wavelength above and one below the isosbestic point of 810 nm—at which deoxy-Hb and oxy-Hb have identical absorption coefficients...
37 KB (4,363 words) - 15:54, 9 July 2024
to identify compounds. Both are forms of vibrational spectroscopy. Isosbestic point – a wavelength where absorption does not change as the reaction proceeds...
34 KB (4,414 words) - 01:56, 4 June 2024
change from red–violet at 25 °C to pale yellow at 150 °C. An isosbestic point (a point where the absorbance curves at all temperatures overlap) indicates...
48 KB (5,347 words) - 05:58, 21 August 2024