In spectroscopy, an isosbestic point is a specific wavelength, wavenumber or frequency at which the total absorbance of a sample does not change during...

maxima) for the individual species. None of the wavelengths may be an isosbestic point for a pair of species. The set of the following simultaneous equations...

4-nitrophenol has a weak absorbance at 405 nm (ε = 0.2 mM−1 cm−1). The isosbestic point for 4-nitrophenol/4-nitrophenoxide is at 348 nm, with ε = 5.4 mM−1...

shifts in neutral pH. Spectroscopic superposition indicated TNP-ATP’s isosbestic point to be 339 nm. At low concentrations of TNP-ATP (≤1 μM), fluorescent...

the maximum absorption and isosbestic points of the indicator. The isosbestic point of GCaMP is the calcium-independent signal, approximately 405-415 nm...

characteristic blue-green color. The acid and basic forms of this dye have an isosbestic point in their UV-Visible spectrum, around 515 nm, indicate that the two...

in situ to give oxidized, ring-opened products. Fluorescein has an isosbestic point (equal absorption for all pH values) at 460 nm. Many derivatives of...

wavelengths are selected, with one wavelength above and one below the isosbestic point of 810 nm—at which deoxy-Hb and oxy-Hb have identical absorption coefficients...

to identify compounds. Both are forms of vibrational spectroscopy. Isosbestic point – a wavelength where absorption does not change as the reaction proceeds...

change from red–violet at 25 °C to pale yellow at 150 °C. An isosbestic point (a point where the absorbance curves at all temperatures overlap) indicates...