ColE1 is a plasmid found in bacteria. Its name derives from the fact that it carries a gene for colicin E1 (the cea gene). It also codes for immunity from...
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also called stringent plasmids, require tighter control of replication. In ColE1 derived plasmids, replication is primarily regulated through a small plasmid-encoded...
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natural plasmids such the ColE1 and pSC101. Each of these plasmids may have its advantages and disadvantages. For example, the ColE1 plasmid and its derivatives...
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(ROM)) is a small dimeric protein responsible for keeping the copy number of ColE1 family and related bacterial plasmids low in E. coli by increasing the speed...
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suitable origin of replication (ori): for example SV40 ori in mammalian cells, ColE1 ori for double-stranded DNA replication, or f1 ori for single-stranded DNA...
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antisense repressor of the replication of some E. coli plasmids, including ColE1. Plasmid replication is usually initiated by RNAII, which acts as a primer...
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commonly used plasmids are based on the ColE1 replicon and are therefore incompatible with each other; in order for a ColE1-based plasmid to coexist with another...
34 KB (4,178 words) - 01:07, 24 March 2024
maintenance in E. coli, such as a functional origin of replication (ori). The ColE1 origin of replication is found in many plasmids. Some vectors also include...
20 KB (2,517 words) - 08:10, 5 October 2023
plasmid ColE1, the asRNA termed RNA I plays an important role in determining the plasmid copy number by controlling replication. The replication of ColE1 relies...
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(May 1980). "Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H". Proceedings of the National Academy of Sciences...
18 KB (2,103 words) - 14:27, 19 July 2024