• A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists...
    14 KB (1,573 words) - 10:45, 9 July 2024
  • Thumbnail for Primer (molecular biology)
    mishybridization. Primers should not easily anneal with other primers in the mixture; this phenomenon can lead to the production of 'primer dimer' products contaminating...
    16 KB (2,112 words) - 01:14, 11 April 2024
  • bands on PCR gels. Primer dimer formation often competes with formation of the DNA fragment of interest, and may be avoided using primers that are designed...
    16 KB (2,030 words) - 10:07, 20 June 2024
  • Thumbnail for Real-time polymerase chain reaction
    all dsDNA PCR products, including nonspecific PCR products (such as primer dimer). This can potentially interfere with, or prevent, accurate monitoring...
    43 KB (5,338 words) - 11:09, 12 July 2024
  • the mixture, are the cause behind the synthesis of by-products such as primer dimer and mis-priming. Mis-priming greatly impedes and reduces the efficiency...
    22 KB (2,750 words) - 14:49, 30 April 2023
  • Thumbnail for Polymerase chain reaction
    that utilizes primers with a 3' extension block that can be removed by a thermostable RNase HII enzyme. This system reduces primer-dimers and allows for...
    89 KB (11,117 words) - 12:33, 8 July 2024
  • Thumbnail for Taq polymerase
    facilitates high specificity of the primers and reduces the production of nonspecific products, such as primer dimer. Also, use of a thermostable polymerase...
    22 KB (2,604 words) - 06:32, 1 June 2024
  • added to view LAMP in real-time. However, in the late amplification, primer-dimer amplification may contribute to a false positive signal. The use of inorganic...
    26 KB (3,025 words) - 15:17, 1 July 2024
  • Thumbnail for Reverse transcription polymerase chain reaction
    using DNA binding dyes such as SYBR Green since the elimination of primer-dimers can be achieved through a simple change in the melting temperature....
    43 KB (5,536 words) - 07:35, 12 July 2024
  • Thumbnail for RNase H-dependent PCR
    reduced primer dimer formation, detection of alternative splicing variants, ability to perform multiplex PCR with higher numbers of PCR primers, and the...
    5 KB (596 words) - 01:29, 3 December 2023