A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists...
14 KB (1,573 words) - 10:45, 9 July 2024
mishybridization. Primers should not easily anneal with other primers in the mixture; this phenomenon can lead to the production of 'primer dimer' products contaminating...
16 KB (2,112 words) - 01:14, 11 April 2024
bands on PCR gels. Primer dimer formation often competes with formation of the DNA fragment of interest, and may be avoided using primers that are designed...
16 KB (2,030 words) - 10:07, 20 June 2024
all dsDNA PCR products, including nonspecific PCR products (such as primer dimer). This can potentially interfere with, or prevent, accurate monitoring...
43 KB (5,338 words) - 11:09, 12 July 2024
Hot start PCR (section Modified primers)
the mixture, are the cause behind the synthesis of by-products such as primer dimer and mis-priming. Mis-priming greatly impedes and reduces the efficiency...
22 KB (2,750 words) - 14:49, 30 April 2023
Polymerase chain reaction (redirect from Single Specific Primer-PCR)
that utilizes primers with a 3' extension block that can be removed by a thermostable RNase HII enzyme. This system reduces primer-dimers and allows for...
89 KB (11,117 words) - 12:33, 8 July 2024
facilitates high specificity of the primers and reduces the production of nonspecific products, such as primer dimer. Also, use of a thermostable polymerase...
22 KB (2,604 words) - 06:32, 1 June 2024
added to view LAMP in real-time. However, in the late amplification, primer-dimer amplification may contribute to a false positive signal. The use of inorganic...
26 KB (3,025 words) - 15:17, 1 July 2024
using DNA binding dyes such as SYBR Green since the elimination of primer-dimers can be achieved through a simple change in the melting temperature....
43 KB (5,536 words) - 07:35, 12 July 2024
reduced primer dimer formation, detection of alternative splicing variants, ability to perform multiplex PCR with higher numbers of PCR primers, and the...
5 KB (596 words) - 01:29, 3 December 2023