Shotgun proteomics refers to the use of bottom-up proteomics techniques in identifying proteins in complex mixtures using a combination of high performance...
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Phosphoproteomics Protein production Proteogenomics Proteomic chemistry Secretomics Shotgun proteomics Top-down proteomics Systems biology Yeast two-hybrid system...
77 KB (9,012 words) - 20:46, 9 September 2024
mass spectrometry. The major alternative workflow used in proteomics is called top-down proteomics where intact proteins are purified prior to digestion and/or...
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Peptide spectral library (section Shotgun proteomics)
early years of shotgun proteomics, pioneer investigations suggested that a similar approach might be applicable in shotgun proteomics for peptide/protein...
6 KB (787 words) - 17:51, 27 January 2024
Metaproteomics (also Community Proteomics, Environmental Proteomics, or Community Proteogenomics) is an umbrella term for experimental approaches to study...
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List of mass spectrometry software (category Proteomics)
Specificity in Data-dependent Quantitative Label-free Shotgun Proteomics". Molecular & Cellular Proteomics. 15 (2): 657–668. doi:10.1074/mcp.M115.055897. PMC 4739679...
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John (2014). "Isobaric Labeling-Based Relative Quantification in Shotgun Proteomics". Journal of Proteome Research. 13 (12): 5293–5309. doi:10.1021/pr500880b...
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and finally the resulting peptidic fragments are analyzed using shotgun proteomics methods such as LC-MS/MS or SPS-MS3. If instead a protein fusion is...
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focusing device as a micro-preparative fractionator for two dimensional shotgun proteomics". Analytical Chemistry. 86 (12): 5728–5732. doi:10.1021/ac404180e...
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challenge for bottom up proteomics due to the inability to generate sufficient tryptic peptides for analysis. Top-down proteomics allows for low mass protein...
11 KB (1,363 words) - 15:08, 16 February 2024