• DNA ends refer to the properties of the ends of linear DNA molecules, which in molecular biology are described as "sticky" or "blunt" based on the shape...
    8 KB (1,091 words) - 08:30, 22 February 2024
  • Blunt end may refer to: Sticky and blunt ends The flat end cab of the British Rail Class 91, commonly called the blunt end cab This disambiguation page...
    159 bytes (57 words) - 22:46, 4 July 2018
  • Thumbnail for Recombinant DNA
    chimera. rDNA technology uses palindromic sequences and leads to the production of sticky and blunt ends. The DNA sequences used in the construction of recombinant...
    35 KB (3,893 words) - 18:12, 12 May 2024
  • Thumbnail for Ligation (molecular biology)
    molecular biology research tool due to its capability of joining as sticky as blunt DNA ends. However, procedures for ligation without the use of standard DNA...
    38 KB (4,862 words) - 03:50, 15 May 2024
  • ligate complementary sticky and blunt ends, but blunt-end ligation is inefficient and requires a higher concentration of both DNA and DNA ligase than the...
    12 KB (1,724 words) - 12:15, 10 September 2023
  • relying on sticky ends, the blunt end TOPO vector has blunt ends where the topoisomerase molecules are bound. Commercial kits, such as the Zero Blunt® Cloning...
    4 KB (568 words) - 01:06, 18 June 2024
  • leaves no overhang, called a blunt end. Blunt ends are much less likely to be ligated by a DNA ligase because the blunt end doesn't have the overhanging...
    5 KB (629 words) - 12:27, 17 March 2022
  • ends of other DNA or RNA molecules. Double stranded adapters can be synthesized to have blunt ends to both terminals or to have sticky end at one end...
    2 KB (245 words) - 01:17, 17 January 2024
  • Thumbnail for Cas12a
    protospacer adjacent motif preference, and for creating sticky rather than blunt ends at the cut site. These and other differences may make it more suitable...
    13 KB (1,530 words) - 12:46, 22 March 2024
  • Thumbnail for EcoRV
    The ends are blunt and can be ligated into a blunt cloning site easily but with lower efficiency than sticky ends. The structure of this enzyme, and several...
    5 KB (506 words) - 00:49, 14 June 2024