Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense...
43 KB (5,610 words) - 21:17, 2 May 2024
CRISPR gene editing (redirect from CRISPR-Cas9 gene editing)
on a simplified version of the bacterial CRISPR-Cas9 antiviral defense system. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA)...
170 KB (19,889 words) - 21:09, 14 September 2024
CRISPR (redirect from CRISPR/Cas9-mediated genome editing)
complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes...
128 KB (13,448 words) - 03:26, 19 August 2024
Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide...
55 KB (6,164 words) - 23:47, 3 December 2023
Guide RNA (section CRISPR Cas9)
RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9-endonuclease or other Cas-proteins that cut the double-stranded DNA and thereby...
30 KB (3,628 words) - 15:29, 5 August 2024
Epigenome editing (redirect from FIRE-Cas9)
proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR). Comparing genome-wide epigenetic maps with gene expression...
30 KB (3,420 words) - 09:03, 16 August 2024
Cas12a (section Cas9 vs. Cas12a)
highly targeted modifications of DNA, similar to CRISPR-Cas9 system. Cas12a is distinguished from Cas9 by a its single RuvC endonuclease active site, its 5'...
13 KB (1,530 words) - 21:01, 18 August 2024
CRISPR activation (section dCas9)
on Cas9 (from Type II systems), but other effectors like Cas12a (Type V) have been used as well. Cas9 Endonuclease Dead, also known as dead Cas9 or dCas9...
21 KB (2,672 words) - 09:24, 16 August 2024
CRISPR-associated nuclease such as Cas9 attaches to a tracrRNA–crRNA complex, which guides Cas9 to the invading protospacer sequence. But Cas9 will not cleave the protospacer...
12 KB (1,421 words) - 00:22, 14 December 2022
along with the CRISPR-Cas9 machinery. When the modified organism mates and its DNA mixes with that of its mate, the CRISPR-Cas9 tool cuts the partner's...
47 KB (5,127 words) - 10:04, 8 June 2024