• Thumbnail for Cas9
    Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense...
    43 KB (5,610 words) - 21:17, 2 May 2024
  • Thumbnail for CRISPR gene editing
    on a simplified version of the bacterial CRISPR-Cas9 antiviral defense system. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA)...
    170 KB (19,936 words) - 16:29, 8 August 2024
  • Thumbnail for CRISPR
    complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes...
    128 KB (13,446 words) - 03:31, 12 August 2024
  • Thumbnail for Genome-wide CRISPR-Cas9 knockout screens
    Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide...
    55 KB (6,164 words) - 23:47, 3 December 2023
  • RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9-endonuclease or other Cas-proteins that cut the double-stranded DNA and thereby...
    30 KB (3,628 words) - 15:29, 5 August 2024
  • Thumbnail for Epigenome editing
    Epigenome editing (redirect from FIRE-Cas9)
    proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR). Comparing genome-wide epigenetic maps with gene expression...
    29 KB (3,412 words) - 03:49, 5 August 2024
  • on Cas9 (from Type II systems), but other effectors like Cas12a (Type V) have been used as well. Cas9 Endonuclease Dead, also known as dead Cas9 or dCas9...
    21 KB (2,672 words) - 22:49, 25 April 2024
  • Thumbnail for Cas12a
    modifications of DNA or RNA, similar to the better known CRISPR-Cas9 system. Cas12a is distinguished from Cas9 by a its single RuvC endonuclease active site, its 5'...
    13 KB (1,530 words) - 12:46, 22 March 2024
  • Thumbnail for Gene drive
    along with the CRISPR-Cas9 machinery. When the modified organism mates and its DNA mixes with that of its mate, the CRISPR-Cas9 tool cuts the partner's...
    47 KB (5,127 words) - 10:04, 8 June 2024
  • Thumbnail for Anti-CRISPR
    CRISPR–Cas9, the type of mechanism used in the genetic edition of human cells). A year later, a study confirmed the presence of type II-A CRISPR–Cas9 inhibitors...
    32 KB (3,691 words) - 12:05, 22 March 2024