Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense...
43 KB (5,609 words) - 10:59, 18 September 2024
CRISPR gene editing (redirect from CRISPR-Cas9 gene editing)
on a simplified version of the bacterial CRISPR-Cas9 antiviral defense system. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA)...
170 KB (19,897 words) - 15:07, 10 October 2024
CRISPR (redirect from CRISPR/Cas9-mediated genome editing)
complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes...
128 KB (13,448 words) - 06:38, 14 October 2024
Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide...
55 KB (6,164 words) - 23:47, 3 December 2023
Off-target genome editing (section Cas9 nickases)
as: clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9, transcription activator-like effector nucleases (TALEN), meganucleases,...
53 KB (6,846 words) - 19:05, 8 July 2024
Epigenome editing (redirect from FIRE-Cas9)
proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR). Comparing genome-wide epigenetic maps with gene expression...
30 KB (3,420 words) - 09:03, 16 August 2024
CRISPR activation (section dCas9)
the CRISPR-Cas9 system to enhance the expression of specific genes without altering the underlying DNA sequence. Unlike traditional CRISPR-Cas9, which introduces...
22 KB (2,739 words) - 07:27, 29 September 2024
Guide RNA (section CRISPR Cas9)
RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9-endonuclease or other Cas-proteins that cut the double-stranded DNA and thereby...
30 KB (3,628 words) - 15:29, 5 August 2024
Cas12a (section Cas9 vs. Cas12a)
highly targeted modifications of DNA, similar to CRISPR-Cas9 system. Cas12a is distinguished from Cas9 by a its single RuvC endonuclease active site, its 5'...
13 KB (1,530 words) - 21:01, 18 August 2024
CRISPR–Cas9, the type of mechanism used in the genetic edition of human cells). A year later, a study confirmed the presence of type II-A CRISPR–Cas9 inhibitors...
32 KB (3,691 words) - 06:48, 24 August 2024