Intersectin 2

ITSN2

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
1J3T, 1UDL, 1UE9, 1UFF, 1UHF, 3GF9, 3JZY, 4IIO

Identifiers

ITSN2, PRO2015, SH3D1B, SH3P18, SWA, SWAP, intersectin 2

External IDs

OMIM: 604464; MGI: 1338049; HomoloGene: 22627; GeneCards: ITSN2; OMA:ITSN2 - orthologs

Gene location (Mouse)

Chr.	Chromosome 12 (mouse)^[1]

Band	12\|12 A1.1	Start	4,642,638 bp^[1]
Band	12\|12 A1.1	End	4,763,962 bp^[1]

RNA expression pattern

Human

Mouse (ortholog)

n/a

Top expressed in

zygote

granulocyte

secondary oocyte

iris

ciliary body

spleen

conjunctival fornix

neural layer of retina

retinal pigment epithelium

blood


More reference expression data

Gene ontology
Molecular function	calcium ion binding protein binding metal ion binding
Cellular component	cytoplasm centrosome extracellular exosome
Biological process	positive regulation of dendrite extension cell differentiation regulation of Rho protein signal transduction endocytosis positive regulation of signal transduction regulation of molecular function viral process
Sources:Amigo / QuickGO

Species

Human

Mouse


50618


20403


ENSG00000198399


ENSMUSG00000020640


Q9NZM3


Q9Z0R6

RefSeq (mRNA)


NM_147152 NM_006277 NM_019595


NM_001198968 NM_001198969 NM_011365

RefSeq (protein)

NP_006268 NP_062541 NP_671494 NP_001335110 NP_001335111
NP_001335112 NP_001335113 NP_001335114 NP_001335115


n/a

Location (UCSC)

n/a

Chr 12: 4.64 – 4.76 Mb

PubMed search

^[2]

^[3]

View/Edit Human

View/Edit Mouse

Intersectin-2 is a protein that in humans is encoded by the ITSN2 gene.^[4]^[5]^[6]

This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. Alternatively spliced transcript variants have been found for this gene that encode three distinct isoforms. Additional variants have been found but their full length nature has not been determined.^[6]

Interactions

ITSN2 has been shown to interact with Wiskott-Aldrich syndrome protein.^[5]

References

^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020640 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Pucharcos C, Estivill X, de la Luna S (Aug 2000). "Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis". FEBS Lett. 478 (1–2): 43–51. doi:10.1016/S0014-5793(00)01793-2. PMID 10922467. S2CID 24683452.
^ ^a ^b McGavin MK, Badour K, Hardy LA, Kubiseski TJ, Zhang J, Siminovitch KA (Dec 2001). "The intersectin 2 adaptor links Wiskott Aldrich Syndrome protein (WASp)-mediated actin polymerization to T cell antigen receptor endocytosis". J Exp Med. 194 (12): 1777–87. doi:10.1084/jem.194.12.1777. PMC 2193569. PMID 11748279.
^ ^a ^b "Entrez Gene: ITSN2 intersectin 2".

Further reading

Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Sparks AB, Hoffman NG, McConnell SJ, et al. (1998). "Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins". Nat. Biotechnol. 14 (6): 741–4. doi:10.1038/nbt0696-741. PMID 9630982. S2CID 11166026.
Nagase T, Ishikawa K, Kikuno R, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (5): 337–45. doi:10.1093/dnares/6.5.337. PMID 10574462.
Pucharcos C, Casas C, Nadal M, et al. (2001). "The human intersectin genes and their spliced variants are differentially expressed". Biochim. Biophys. Acta. 1521 (1–3): 1–11. doi:10.1016/s0167-4781(01)00276-7. PMID 11690630.
Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Chini CC, Chen J (2003). "Human claspin is required for replication checkpoint control". J. Biol. Chem. 278 (32): 30057–62. doi:10.1074/jbc.M301136200. PMID 12766152.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Brill LM, Salomon AR, Ficarro SB, et al. (2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Anal. Chem. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
Zhang Y, Wolf-Yadlin A, Ross PL, et al. (2005). "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules". Mol. Cell. Proteomics. 4 (9): 1240–50. doi:10.1074/mcp.M500089-MCP200. PMID 15951569.
Nishimura T, Yamaguchi T, Tokunaga A, et al. (2006). "Role of numb in dendritic spine development with a Cdc42 GEF intersectin and EphB2". Mol. Biol. Cell. 17 (3): 1273–85. doi:10.1091/mbc.E05-07-0700. PMC 1382316. PMID 16394100.
Seifert M, Ampofo C, Mehraein Y, et al. (2007). "Expression analysis of human intersectin 2 gene (ITSN2) minor splice variants showing differential expression in normal human brain". Oncol. Rep. 17 (5): 1207–11. doi:10.3892/or.17.5.1207. PMID 17390067.
Lim CS, Seet BT, Ingham RJ, et al. (2007). "The K15 protein of Kaposi's sarcoma-associated herpesvirus recruits the endocytic regulator intersectin 2 through a selective SH3 domain interaction". Biochemistry. 46 (35): 9874–85. doi:10.1021/bi700357s. PMID 17696407.

PDB gallery

1j3t: Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)
1udl: The solution structure of the fifth SH3 domain of intersectin 2 (KIAA1256)
1ue9: Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)
1uff: Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)
1uhf: Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)

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