Alternative splicing - Simple English Wikipedia, the free encyclopedia

Alternative splicing allows DNA to code for more than one protein. It varies the exon make-up of the messenger RNA.

In alternative splicing the exons of the pre-messenger RNA produced by transcription are reconnected in different ways during RNA splicing.

This produces different mature messenger RNAs from the same gene. They get translated into different proteins. Thus, a single gene may code for multiple proteins.^[1]

Alternative splicing is normal in eukaryotes. It greatly increases the diversity of proteins that can be encoded by the genome.^[1] In humans, ~95% of multiexonic genes are alternatively spliced.^[2][3][4]

There are various kinds of alternative splicing: the most common is exon skipping. An exon may be included in mRNAs under some conditions or in particular tissues, and omitted from the mRNA in others.^[1] There are splicing activators that promote the use of a particular splice site, and splicing repressors that reduce the use of a particular site. New types of alternative splicing are being found.^[4][5]

Abnormal variations in splicing occur in disease. Many human genetic disorders come from splicing variants.^[4] Abnormal splicing variants may also contribute to the development of cancer.^[6][7][8] High-throughput sequencing of RNA can for example be used to measure the genome-scale amount of deviating alternative splicing, such as in a cohort of colorectal cancers, where high amount of aberrant splicing was associated to poor patient survival.^[9] Non-working splicing products are usually dealt with by post-transcriptional quality control.^[10] That is, they are chopped up by enzymes.

Alternative splicing (the re-combination of different exons) is a major source of genetic diversity in eukaryotes. One particular Drosophila gene (DSCAM) can be alternatively spliced into 38,000 different mRNA.^[11]

• CRISPR