| | District | Senator | Party | Constituency | Began serving |
| | 1st |  | Alanson T. Lincoln | Republican | Washington, Smyth, and city of Bristol | 1908 |
| | 2nd |  | John C. Noel | Republican | Scott, Lee, and Wise | 1904 |
| | 3rd |  | Roland E. Chase | Republican | Buchanan, Dickenson, Russell, and Tazewell | 1908 |
| | 4th |  | John M. Hart | Democratic | Roanoke, Montgomery, and cities of Roanoke and Radford | 1908 |
| | 5th |  | A. Pendleton Strother | Republican | Giles, Bland, Pulaski, and Wythe | 1908 |
| | 6th |  | John M. Parsons | Republican | Carroll, Grayson, and Patrick | 1908 |
| | 7th |  | Floyd W. King | Democratic | Craig, Botetourt, Allegheny, Bath, and city of Clifton Forge | 1908 |
| | 8th |  | George B. Keezell | Republican | Rockingham | 1896
(previously served 1884-1887) |
| | 9th |  | Edward Echols | Democratic | Augusta, Highland, and city of Staunton | 1906
(previously served 1889-1897) |
| | 10th |  | Robert M. Ward | Democratic | Shenandoah, Frederick, and city of Winchester | 1908 |
| | 11th |  | G. Latham Fletcher | Democratic | Fauquier and Loudoun | 1908 |
| | 12th |  | Richard S. Parks | Democratic | Clarke, Page, and Warren | 1908 |
| | 13th |  | F. Wilmer Sims | Democratic | Spotsylvania, Stafford, Louisa, and city of Fredericksburg | 1906 |
| | 14th |  | R. Ewell Thornton | Democratic | Alexandria county, Prince William, Fairfax, and city of Alexandria | 1908 |
| | 15th |  | F. Pendleton Carter | Democratic | Culpeper, Madison, Rappahannock, and Orange | 1908 |
| | 16th |  | John B. Watkins | Democratic | Goochland, Powhatan, and Chesterfield | 1908 |
| | 17th |  | Nathaniel B. Early | Democratic | Albemarle, Greene, and city of Charlottesville | 1908 |
| | 18th |  | Sands Gayle | Democratic | Appomattox, Buckingham, Fluvanna, and Charlotte | 1910 |
| | 19th |  | Aubrey E. Strode | Democratic | Amherst and Nelson | 1906 |
| | 20th |  | Don P. Halsey | Democratic | Campbell and city of Lynchburg | 1908
(previously served 1902-1904) |
| | 21st |  | Henry A. Edmondson | Democratic | Halifax | 1908 |
| | 22nd |  | J. Randolph Tucker | Democratic | Bedford, Rockbridge, and city of Buena Vista | 1908 |
| | 23rd |  | William A. Garrett | Democratic | Pittsylvania, Henry, and city of Danville | 1901 |
| | 24th |  | George T. Rison | Democratic | Pittsylvania and city of Danville | 1904 |
| | 25th |  | James D. Elam | Democratic | Mecklenburg and Brunswick | 1908
(previously served 1901-1904) |
| | 26th |  | Greenville O. McAlexander | Republican | Franklin and Floyd | 1908 |
| | 27th |  | Alexander R. Hobbs | Democratic | Greensville, Sussex, Surry, and Prince George | 1901 |
| | 28th |  | John J. Owen | Democratic | Nottoway, Amelia, Lunenburg, Prince Edward, and Cumberland | 1910 |
| | 29th |  | Charles T. Lassiter | Democratic | Dinwiddie and city of Petersburg | 1906 |
| | 30th |  | Edward E. Holland | Democratic | Isle of Wight, Southampton, and Nansemond | 1908 |
| | 31st |  | William W. Sale | Democratic | Norfolk city | 1908 |
| | 32nd |  | Charles U. Gravatt | Democratic | Caroline, Hanover, and King William | 1908 |
| | 33rd |  | John A. Lesner | Democratic | Norfolk county and city of Portsmouth | 1908 |
| | 34th |  | C. Harding Walker | Democratic | King George, Richmond, Westmoreland, Lancaster, and Northumberland | 1899 |
| | 35th |  | T. Ashby Wickham | Democratic | Henrico, New Kent, Charles City, James City, and city of Williamsburg | 1906 |
| | 36th |  | Saxon W. Holt | Democratic | Elizabeth City, York, Warwick, and city of Newport News | 1904 |
| | 37th |  | Ben T. Gunter | Democratic | Accomac, Northampton, and Princess Anne | 1904 |
| | 38th |  | Elben C. Folkes | Democratic | Richmond city | 1908 |
| |  | Arthur C. Harman | Democratic | 1904 |
| | 39th |  | John R. Saunders | Democratic | King and Queen, Middlesex, Essex, Gloucester, and Mathews | 1908 |
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