| District | Senator | Party | Constituency | Began serving |
| 1st | | J. Cloyd Byars | Republican | Washington, Smyth, and city of Bristol | 1901 |
| 2nd | | Robert P. Bruce | Democratic | Scott, Lee, and Wise | 1899 |
| 3rd | | John N. Harman | Republican | Buchanan, Dickenson, Russell, and Tazewell | 1901 |
| 4th | | Edward Lyle | Democratic | Montgomery, Roanoke, Craig, and city of Roanoke | 1899 |
| 5th | | Peyton F. St. Clair | Democratic | Giles, Bland, Pulaski, and Wythe | 1901 |
| 6th | | Edwin J. Harvey | Democratic | Carroll, Grayson, and Patrick | 1899 |
| 7th | | George A. Revercomb | Republican | Rockbridge, Botetourt, Allegheny, Bath, and Highland | 1901 |
| 8th | | George B. Keezell | Democratic | Rockingham | 1896 (previously served 1884-1887) |
| 9th | | John N. Opie | Democratic | Augusta and city of Staunton | 1897 |
| 10th | | Silas L. Lupton | Democratic | Frederick and Shenandoah | 1899 |
| 11th | | George T. Ford | Democratic | Fauquier and Loudoun | 1901 |
| 12th | | Thomas D. Gold | Democratic | Clarke, Page, and Warren | 1899 |
| 13th | | Gustavus M. Wallace | Democratic | Spotsylvania, Stafford, Louisa, and city of Fredericksburg | 1897 |
| 14th | | Stephen R. Donohoe | Democratic | Alexandria, Fairfax, Prince William, and city of Alexandria | 1899 |
| 15th | | George S. Shackelford | Democratic | Culpeper, Rappahannock, Madison, and Orange | 1901 |
| 16th | | Philip V. Cogbill | Democratic | Powhatan, Goochland, and Chesterfield | 1899 |
| 17th | | John S. Chapman | Democratic | Albemarle, Greene, and city of Charlottesville | 1901 |
| 18th | | Frank C. Moon | Democratic | Buckingham, Fluvanna, and Appomattox | 1901 |
| 19th | | Bland Massie | Democratic | Amherst and Nelson | 1897 |
| 20th | | Carter Glass | Democratic | Campbell and city of Lynchburg | 1899 |
| 21st | | William P. Barksdale | Democratic | Halifax | 1897 |
| 22nd | | Graham Claytor | Democratic | Bedford | 1899 |
| 23rd | | William A. Garrett | Democratic | Pittsylvania, Henry, and city of Danville | 1901 |
| 24th | | Joseph Whitehead | Democratic | Pittsylvania and city of Danville | 1899 |
| 25th | | James N. Hutcheson | Democratic | Charlotte and Mecklenburg | 1901 |
| 26th | | James A. Dinwiddie | Republican | Floyd and Franklin | 1899 |
| 27th | | Alexander R. Hobbs | Democratic | Greensville, Sussex, Surry, and Prince George | 1901 |
| 28th | | William Hodges Mann | Democratic | Lunenburg, Nottoway, and Brunswick | 1899 |
| 29th | | William B. McIlwaine | Democratic | Dinwiddie and city of Petersburg | 1893 |
| 30th | | Asa D. Watkins | Democratic | Amelia, Cumberland, and Prince Edward | 1899 |
| 31st | | William W. Sale | Democratic | Norfolk city and Princess Anne | 1901 |
| 32nd | | William Shands | Democratic | Southampton, Isle of Wight, and Nansemond | 1899 |
| 33rd | | E. Finley Cromwell | Democratic | Portsmouth and Norfolk county | 1901 |
| 34th | | Henry T. Wickham | Democratic | Hanover and Caroline | 1889 |
| 35th | | George Wayne Anderson | Democratic | Henrico and city of Richmond | 1901 |
| | Julian Bryant | Democratic | 1901 |
| 36th | | C. Harding Walker | Democratic | King George, Richmond, Westmoreland, Northumberland, and Lancaster | 1899 |
| 37th | | George W. LeCato | Democratic | Accomac and Northampton | 1893 |
| 38th | | D. Gardiner Tyler | Democratic | Elizabeth City, Warwick, York, James City, Charles City, New Kent, King William, and cities of Williamsburg and Newport News | 1899 (previously served 1891-1893) |
| 39th | | J. Boyd Sears | Democratic | King and Queen, Middlesex, Gloucester, Essex, and Mathews | 1901 |